Active Recombinant Human GDF1

• Cat.No.: GDF1-715H
• Product Overview: Recombinant Human GDF1 (Accession # NP_001483) Asp254-Arg372 was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO cells
• Species: Human
• Predicted N Terminal: Asp254
• Form: Lyophilized from a 0.2 µm filtered solution in HCl with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:653.50 ng/mL of Recombinant Human GDF-1 can effectively induce Smad2 phosphorylation.
• Molecular Mass: Recombinant Human GDF1 has a calculated MW of 12.6 kDa (monomer). In SDS-PAGE migrates as 13 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: ITGAV integrin, alpha V [ Homo sapiens ]
• Official Symbol: ITGAV
• Synonyms: ITGAV; integrin, alpha V; antigen identified by monoclonal L230 , integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) , MSK8, vitronectin receptor , VNRA, VTNR; integrin alpha-V; CD51; integrin alphaVbeta3; vitronectin receptor subunit alpha; antigen identified by monoclonal L230; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; VNRA; VTNR; DKFZp686A08142
• Gene ID: 3685
• mRNA Refseq: NM_001144999
• Protein Refseq: NP_001138471
• MIM: 193210
• UniProt ID: P06756
• Chromosome Location: 2q31-q32