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Recombinant Human TNC, His-tagged

Cat.No.: TNC-734H
Product Overview: Recombinant Human TNC (Accession # NP_002151) Gly23-Pro625, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO cells
Species: Human
Tag: His
Predicted N Terminal: Gly23
Form: Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity: Measured by the ability of the immobilized protein to block Fibronectin-mediated adhesion of NIH-3T3 mouse embryonic fibroblast cells.rhTenascin-C immobilized at 15 µg/mL, in the presence of 0.1 µg/mL human Fibronectin, will block approximately 70% - 90% NIH3/T3 cell adhesion (5 x 10^4 cells/well, 100 µL/well).
Molecular Mass: Recombinant Human TNC, His-tagged has a calculated MW of 65.3 kDa. In SDS-PAGE migrates as 97 kDa, reducing conditions.
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: TNC tenascin C [ Homo sapiens ]
Official Symbol: TNC
Synonyms: TNC; tenascin C; hexabrachion (tenascin C, cytotactin) , HXB; tenascin; hexabrachion (tenascin); MGC167029; TN; GP 150-225; cytotactin; neuronectin; myotendinous antigen; tenascin-C isoform 14/AD1/16; glioma-associated-extracellular matrix antigen; GP; JI; HXB; GMEM; TN-C; 150-225;
Gene ID: 3371
mRNA Refseq: NM_002160
Protein Refseq: NP_002151
MIM: 187380
UniProt ID: P24821
Chromosome Location: 9q32-q34

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