||Recombinant Human ITGA2&ITGB1 (Accession # NP_002194 (Integrin alpha 2) & P05556 (Integrin beta 1)) was produced in Chinese Hamster Ovary cell line, CHO-derived.
|Predicted N Terminal:
||Tyr30 ( alpha 2) & No results obtained, Gln21 predicted ( beta 1)
||Lyophilized from a 0.2 µm filtered solution in PBS.
||Measured by its binding ability in a functional ELISA.When bovine collagen-II is coated at 10 µg/mL, Recombinant Human Integrin alpha 2 beta 1 binds with an apparent KD <1nM.
||Recombinant Human ITGA2&ITGB1 has a calculated MW of 126 kDa ( alpha 2) & 83.3 kDa ( beta 1). In SDS-PAGE migrates as 140-150 kDa ( alpha 2) & 120-130 kDa ( beta 1), reducing conditions.
||>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).