Active Recombinant Human GPNMB, Fc Chimera

• Cat.No.: GPNMB-747H
• Product Overview: Recombinant Human GPNMB (Accession # NP_002501) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc Chimera
• Predicted N Terminal: Lys23
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to induce pro-MMP-9 secretion by NHLF human normal lung fibroblastsThe ED50 for this effect is typically 2.5 - 10 µg/mL. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human GPNMB, Fc Chimera has a calculated MW of 78.6 kDa (monomer). In SDS-PAGE migrates as 115-125 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: GPNMB glycoprotein (transmembrane) nmb [ Homo sapiens ]
• Official Symbol: GPNMB
• Synonyms: GPNMB; glycoprotein (transmembrane) nmb; transmembrane glycoprotein NMB; glycoprotein NMB; glycoprotein nmb like protein; HGFIN; NMB; osteoactivin; transmembrane glycoprotein; glycoprotein nmb-like protein; transmembrane glycoprotein HGFIN
• Gene ID: 10457
• mRNA Refseq: NM_001005340
• Protein Refseq: NP_001005340
• MIM: 604368
• UniProt ID: Q14956
• Chromosome Location: 7p