Recombinant Human EGFR, His&Avi-tagged, Biotinylated EGFR-285H

Recombinant Human EGFR, His&Avi-tagged, Biotinylated

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Recombinant Human EGFR, His&Avi-tagged, Biotinylated

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Cat.No. : EGFR-285H
Product Overview : Recombinant Human EGFR(Leu 25 - Ser 645), fused with His&Avi-tag at C-terminal, Biotinylated, was produced in human 293 cells (HEK293).
Source : HEK293
Species : Human
Tag : His&Avi
Predicted N Terminal : Leu 25
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized CetuxiMab at 2 μg/mL (100 μL/well) can bind Biotinylated rh EGFR, Avi Tagged with a linear range of 0.002-0.2 μg/mL.
Molecular Mass : Have a calculated MW of 71.2 kDa. The predicted N-terminus is Leu 25. DTT-reduced Protein migrates as 110-115 kDa in SDS-PAGE due to glycosylation.
Endotoxin : Less than 1.0 EU per μg of the Biotinylated rh EGFR /ErbB1, His.Avi Tag by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Applications : By co-expression with biotin ligase (BirA enzyme), Biotinylated Human EGFR /ErbB1, His.Avi Tag was biotinylated in vivo for detection in ELISA, dot blot or western blot using streptavidin or avidin-conjugated probes.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).
Gene Name : EGFR epidermal growth factor receptor [ Homo sapiens ]
Official Symbol : EGFR
Synonyms : ERBB; HER1; mENA; ERBB1; PIG61; NISBD2; epidermal growth factor receptor; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; erb-b2 receptor tyrosine kinase 1; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1
Gene ID : 1956
mRNA Refseq : NM_005228
Protein Refseq : NP_005219
MIM : 131550
UniProt ID : P00533
Chromosome Location : 7p12
Pathway : AGE/RAGE pathway, organism-specific biosystem; Adherens junction, organism-specific biosystem; Arf6 signaling events, organism-specific biosystem
Function : ATP binding; MAP kinase kinase kinase activity; chromatin binding

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