Recombinant Human EGFR, His&Avi-tagged, Biotinylated


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Recombinant Human EGFR, His&Avi-tagged, Biotinylated

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Cat.No. : EGFR-285H
Product Overview : Recombinant Human EGFR(Leu 25 - Ser 645), fused with His&Avi-tag at C-terminal, Biotinylated, was produced in human 293 cells (HEK293).
Source : HEK293
Species : Human
Tag : His&Avi
Predicted N Terminal : Leu 25
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized CetuxiMab at 2 μg/mL (100 μL/well) can bind Biotinylated rh EGFR, Avi Tagged with a linear range of 0.002-0.2 μg/mL.
Molecular Mass : Have a calculated MW of 71.2 kDa. The predicted N-terminus is Leu 25. DTT-reduced Protein migrates as 110-115 kDa in SDS-PAGE due to glycosylation.
Endotoxin : Less than 1.0 EU per μg of the Biotinylated rh EGFR /ErbB1, His.Avi Tag by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Applications : By co-expression with biotin ligase (BirA enzyme), Biotinylated Human EGFR /ErbB1, His.Avi Tag was biotinylated in vivo for detection in ELISA, dot blot or western blot using streptavidin or avidin-conjugated probes.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).
Gene Name : EGFR epidermal growth factor receptor [ Homo sapiens ]
Official Symbol : EGFR
Synonyms : ERBB; HER1; mENA; ERBB1; PIG61; NISBD2; epidermal growth factor receptor; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; erb-b2 receptor tyrosine kinase 1; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1
Gene ID : 1956
mRNA Refseq : NM_005228
Protein Refseq : NP_005219
MIM : 131550
UniProt ID : P00533
Chromosome Location : 7p12
Pathway : AGE/RAGE pathway, organism-specific biosystem; Adherens junction, organism-specific biosystem; Arf6 signaling events, organism-specific biosystem
Function : ATP binding; MAP kinase kinase kinase activity; chromatin binding
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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