Active Recombinant Human CA2

• Cat.No.: CA2-219H
• Product Overview: Recombinant Human CA2 was expressed in E. coli.

Specification

• Description: Carbonic anhydrase is a zinc metalloenzyme that catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital physiological and pathological processes such as pH and CO2 homeostasis, transport of bicarbonate and CO2, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. It is required for renal acidification. Its absence can lead to osteoporosis, renal tubular acidosis, and cerebral calcification. Therefore, this enzyme is an important target for inhibitors with clinical applications in glaucoma, epilepsy and Parkinson’s disease. In addition, it is being explored as a potential target for obesity and cancer. CAII has a molecular mass of approximately 30 kDa and is primarily present in type II pneumocytes. Due to this unique location it has been speculated that CAII is involved in pulmonary functions such as regulation of fluid secretion and facilitation of CO2 elimation. Sulfonamides, sulfamates and sulfamides are potent inhibitors of CA.
• Source: E. coli
• Species: Human
• Form: Supplied as a solution in 20 mM Tris, pH 7.5, with 150 mM NaCl.
• Bio-activity: ≥5,000 units/mg protein
• Unit Definition: One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)
• Applications: Human carbonic anhydrase II has been used in a study to assess quantitative imaging of mitochondrial and cytosolic free zinc levels in an in vitro model of ischemia/reperfusion. Human carbonic anhydrase II has also been used in a study to investigate a new scrubber concept for catalytic CO2 hydration.
• Storage: Store at −20°C
• Concentration: 800 - 1000 μg/Ml
• Warning: FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Gene Information

• Gene Name: CA2 carbonic anhydrase II [ Homo sapiens ]
• Official Symbol: CA2
• Synonyms: CAC; CAII; Car2; CA-II; HEL-76; carbonic anhydrase 2; carbonate dehydratase II; carbonic anhydrase B; carbonic anhydrase C; carbonic dehydratase; epididymis luminal protein 76
• Gene ID: 760
• mRNA Refseq: NM_000067
• Protein Refseq: NP_000058
• MIM: 611492
• UniProt ID: P00918
• Chromosome Location: 8q22
• Pathway: Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem; Collecting duct acid secretion, conserved biosystem
• Function: arylesterase activity; carbonate dehydratase activity; protein binding