Active Recombinant Human VEGFB protein, His-tagged

• Cat.No.: VEGFB-17H
• Product Overview: Recombinant Human VEGFB protein(Pro 22 - Ala 207), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Pro 22 - Ala 207
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human VEGF-B, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF R1 with a linear range of 2-32 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 20.3 kDa. The protein migrates as 16 kDa and 34-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: VEGFB vascular endothelial growth factor B [ Homo sapiens ]
• Official Symbol: VEGFB
• Synonyms: VEGFB; vascular endothelial growth factor B; VRF; VEGFL; VEGF-related factor
• Gene ID: 7423
• mRNA Refseq: NM_001243733
• Protein Refseq: NP_001230662
• MIM: 601398
• UniProt ID: P49765