Recombinant Human VEGFB protein, His-tagged VEGFB-17H

Recombinant Human VEGFB protein, His-tagged

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Recombinant Human VEGFB protein, His-tagged

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Cat.No. : VEGFB-17H
Product Overview : Recombinant Human VEGFB protein (Pro22-Ala207), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).
Description : Vascular endothelial growth factor B (VEGFB) is also known as VEGF-related factor (VRF), is a secreted O-glycosylated protein, which belongs to the PDGF/VEGF growth factor family. VEGFB is expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas. VEGFB is growth factor for endothelial cells. VEGF-B seems to play a role only in the maintenance of newly formed blood vessels during pathological conditions. VEGF-B also plays an important role on several types of neurons. It is important for the protection of neurons in the retina and the cerebral cortex during stroke and of motoneurons during motor neuron diseases such as amyotrophic lateral sclerosis.VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 20.3 kDa. The protein migrates as 19 kDa and 32-40 kDa under reducing (R) condition (SDS-PAGE).
Protein length : 186
Endotoxin : Less than 1.0 EU per ug by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of VEGFB-17H :

Human VEGFB, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity of VEGFB-17H :

Immobilized Human VEGF-B, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF R1 with a linear range of 2-32 ng/mL (QC tested).

Gene Name : VEGFB
Official Symbol : VEGFB
Synonyms : VEGFB; vascular endothelial growth factor B; VRF; VEGFL; VEGF-related factor
Gene ID : 7423
mRNA Refseq : NM_001243733
Protein Refseq : NP_001230662
MIM : 601398
UniProt ID : P49765

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