Recombinant Human PGF protein, His-tagged PGF-29H

Recombinant Human PGF protein, His-tagged

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Recombinant Human PGF protein, His-tagged

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Cat.No. : PGF-29H
Product Overview : Recombinant Human PGF protein (Leu19-Arg170), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).
Description : Placental growth factor (PGF) is also known as vascular endothelial growth factor-related protein, PLGF and PlGF2, is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. The main source of PGF during pregnancy is the placental trophoblast. PGF is also expressed in many other tissues, including the villous trophoblast. PGF is actived in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. PlGF2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.
Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from 0.22 um filtered solution in 100 mM Acetic Acid, pH2.0, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 20.2 kDa. The protein migrates as 28-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : 152
Endotoxin : Less than 1.0 EU per ug by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of PGF-29H :

Human PLGF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA of PGF-29H :

Immobilized Rhesus macaque VEGFR1, Mouse IgG2a Fc Tag, low endotoxin at 5 μg/mL (100 μL/well) can bind Human PLGF, His Tag with a linear range of 5-40 ng/mL (QC tested).

Immobilized Human PLGF, His Tag at 1 μg/mL (100 μL/well) can bind Biotinylated Human VEGF R1, His,Avitag with a linear range of 2-20 ng/mL (Routinely tested).

Gene Name : PGF
Official Symbol : PGF
Synonyms : PGF; placental growth factor; PGFL, placental growth factor, vascular endothelial growth factor related protein , placental growth factor like; placenta growth factor; D12S1900; PlGF; PLGF; PlGF 2; SHGC 10760; placental growth factor-like; placental growth factor, vascular endothelial growth factor-related protein; PGFL; PlGF-2; SHGC-10760
Gene ID : 5228
mRNA Refseq : NM_001207012
Protein Refseq : NP_001193941
MIM : 601121
UniProt ID : P49763

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