Recombinant Human UBE2A Protein, His-tagged UBE2A-530H

Recombinant Human UBE2A Protein, His-tagged

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Recombinant Human UBE2A Protein, His-tagged

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Cat.No. : UBE2A-530H
Product Overview : Recombinant Human UBE2A (Accession # NP_003327), fused with an N-terminal 6-His tag, was produced in E. coli.
Source : E. coli
Species : Human
Tag : His
Form : X mg/ml (XµM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Bio-activity : Recombinant Human His6-HR6A/UBE2A is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-HR6A/UBE2A concentration of 0.1-1 µM.
Molecular Mass : Recombinant Human UBE2A, His-tagged has a calculated MW of 19 kDa.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 10 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : UBE2A ubiquitin-conjugating enzyme E2A [ Homo sapiens ]
Official Symbol : UBE2A
Synonyms : UBE2A; ubiquitin-conjugating enzyme E2A; ubiquitin conjugating enzyme E2A (RAD6 homolog); ubiquitin-conjugating enzyme E2 A; HHR6A; RAD6A; UBC2; HR6A; RAD6 homolog A; ubiquitin-protein ligase A; ubiquitin carrier protein A; ubiquitin-conjugating enzyme E2A (RAD6 homolog); MRXSN; MRXS30;
Gene ID : 7319
mRNA Refseq : NM_003336
Protein Refseq : NP_003327
MIM : 312180
UniProt ID : P49459
Chromosome Location : Xq24

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