Active Recombinant Human FZD5 Protein, Fc Chimera

• Cat.No.: FZD5-536H
• Product Overview: Recombinant Human FZD5 (Accession # NP_003459), fused with , was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Human
• Tag: Fc
• Predicted N Terminal: Ala27 and Ser28
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA.In a 100 µL reaction mixture containing biotinylated recombinant mouse Wnt-5a at 100 ng/mL and Recombinant Human Frizzled-5 Fc Chimera dilutions, the concentration of Recombinant Human Frizzled-5 Fc Chimera that produces 50% of the maximal binding response is found to be approximately 5-20 ng/mL. Optimal concentrations should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human FZD5, Fc Chimera has a calculated MW of 50.1 kDa (monomer). In SDS-PAGE migrates as 60-68 kDa, reducing conditons.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: FZD5 frizzled family receptor 5 [ Homo sapiens ]
• Official Symbol: FZD5
• Synonyms: FZD5; frizzled family receptor 5; C2orf31, chromosome 2 open reading frame 31 , frizzled (Drosophila) homolog 5 , frizzled 5, seven transmembrane spanning receptor , frizzled homolog 5 (Drosophila); frizzled-5; DKFZP434E2135; HFZ5; fz-5; fzE5; Wnt receptor; seven-transmembrane receptor frizzled-5; frizzled 5, seven transmembrane spanning receptor; C2orf31; MGC129692; DKFZp434E2135
• Gene ID: 7855
• mRNA Refseq: NM_003468
• Protein Refseq: NP_003459
• MIM: 601723
• UniProt ID: Q13467
• Chromosome Location: 2q33.3