Active Recombinant Human CHST1 Protein, His-tagged

• Cat.No.: CHST1-539H
• Product Overview: Recombinant Human CHST1 (Accession # NP_003645) Arg24-Ser411, fused with an N-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: Arg24-Ser411
• Predicted N Terminal: His
• Form: Supplied as a 0.2 µm filtered solution in Tris-HCl and NaCl.
• Bio-activity: Measured by its ability to transfer sulfate from PAPS to alpha -Lactose.The specific activity is >500 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human CHST1, His-tagged has a calculated MW of 45 kDa. In SDS-PAGE migrates as 58 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: CHST1 carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 [ Homo sapiens ]
• Official Symbol: CHST1
• Synonyms: CHST1; carbohydrate (keratan sulfate Gal-6) sulfotransferase 1; carbohydrate sulfotransferase 1; C6ST; KSGal6ST; carbohydrate (chondroitin 6/keratan) sulfotransferase 1; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1; KSST; GST-1; KS6ST
• Gene ID: 8534
• mRNA Refseq: NM_003654
• Protein Refseq: NP_003645
• MIM: 603797
• UniProt ID: O43916
• Chromosome Location: 11p11.2