Recombinant Human CD74 Protein, HA-tagged CD74-546H

Recombinant Human CD74 Protein, HA-tagged

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Recombinant Human CD74 Protein, HA-tagged

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Cat.No. : CD74-546H
Product Overview : Recombinant Human CD74 (Accession # NP_004346) Gln73-Met232, fused with an N-terminal HA (YPYDVPDYA) tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source : CHO Cells
Species : Human
Tag : HA
Predicted N Terminal : Tyr
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells.When 5 x 10^4 cells per well are added to Recombinant Human CD74 coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is typically 0.6 - 3 µg/mL.Optimal dilutions should be determined by each laboratory for each application.
Molecular Mass : Recombinant Human CD74, HA-tagged has a calculated MW of 19.3 kDa. In SDS-PAGE migrates as 30-35 kDa, reducing conditions.
Protein length : Gln73-Met232
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 100 µg/mL in 4 mM HCl containing 0.1% bovine serum albumin.
Gene Name : CD74 CD74 molecule, major histocompatibility complex, class II invariant chain [ Homo sapiens ]
Official Symbol : CD74
Synonyms : CD74; CD74 molecule, major histocompatibility complex, class II invariant chain; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen associated) , DHLAG; HLA class II histocompatibility antigen gamma chain; gamma chain of class II antigens; HLA DR gamma; Ia associated invariant chain; MHC HLA DR gamma chain; p33; HLA-DR-gamma; MHC HLA-DR gamma chain; Ia-associated invariant chain; HLA-DR antigens-associated invariant chain; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); II; DHLAG; HLADG; Ia-GAMMA; FLJ98970;
Gene ID : 972
mRNA Refseq : NM_001025158
Protein Refseq : NP_001020329
MIM : 142790
UniProt ID : P04233
Chromosome Location : 5q31-q33

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