Active Recombinant Human EPHA3 Protein, Fc Chimera

• Cat.No.: EPHA3-559H
• Product Overview: Recombinant Human EPHA3 (Accession # NP_005224), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Predicted N Terminal: Glu21
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA.When Recombinant Human (rh) EphA3 Fc Chimera is coated at 2 µg/mL (100 µL/well), the concentration of biotinylayed rhEphrin A5 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 5 - 25 ng/mL.
• Molecular Mass: Recombinant Human EPHA3, Fc Chimera has a calculated MW of 85.4 kDa (monomer). In SDS-PAGE migrates as 100-110 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 200 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: EPHA3 EPH receptor A3 [ Homo sapiens ]
• Official Symbol: EPHA3
• Synonyms: EPHA3; EPH receptor A3; EphA3 , ETK, ETK1, TYRO4; ephrin type-A receptor 3; HEK; HEK4; EPH-like kinase 4; human embryo kinase 1; eph-like tyrosine kinase 1; TYRO4 protein tyrosine kinase; tyrosine-protein kinase receptor ETK1; EK4; ETK; ETK1; TYRO4
• Gene ID: 2042
• mRNA Refseq: NM_005233
• Protein Refseq: NP_005224
• MIM: 179611
• UniProt ID: P29320
• Chromosome Location: 3p11.2