Active Recombinant Mouse Nid2 Protein, His-tagged

• Cat.No.: Nid2-612M
• Product Overview: Recombinant Mouse Nid2 (Accession # NP_032721) Leu31-Lys1403, fused with an N-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Not obtained, predicted His-tag
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells.When 5 x 10^4 cells/well are added to Recombinant Mouse Nidogen-2 coated plates (10 µg/mL with 100 µL/well), >40% will adhere after one hour at 37 °C. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Mouse Nid2, His-tagged has a calculated MW of 151.5 kDa. In SDS-PAGE migrates as 170-185 kDa, reducing conditions.
• Protein length: Leu31-Lys1403
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Nid2 nidogen 2 [ Mus musculus ]
• Official Symbol: Nid2
• Synonyms: NID2; nidogen 2; nidogen-2; entactin 2; Ly111; NID-2; AW547149; entactin-2
• Gene ID: 18074
• mRNA Refseq: NM_008695
• Protein Refseq: NP_032721