Active Recombinant Mouse Wnt4

• Cat.No.: Wnt4-622M
• Product Overview: Recombinant Mouse Wnt4 (Accession # NP_033549) Ser23-Arg351, fused with , was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Mouse
• Predicted N Terminal: Ser23
• Form: Lyophilized from a 0.2 µm filtered solution in PBS, NaCl, EDTA and CHAPS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351.The ED50 for this effect is 15-60 ng/mL.
• Molecular Mass: Recombinant Mouse Wnt4 has a calculated MW of 36.6 kDa. In SDS-PAGE migrates as 43 kDa, reducing conditions.
• Protein length: Ser23-Arg351
• Purity: >60%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Wnt4 wingless-related MMTV integration site 4 [ Mus musculus ]
• Official Symbol: Wnt4
• Synonyms: WNT4; wingless-related MMTV integration site 4; protein Wnt-4; signal molecule; Wnt-4; MGC123964
• Gene ID: 22417
• mRNA Refseq: NM_009523
• Protein Refseq: NP_033549