Recombinant Mouse Cd47 protein, Fc-tagged Cd47-645M

Recombinant Mouse Cd47 protein, Fc-tagged


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Recombinant Mouse Cd47 protein, Fc-tagged

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Cat.No. : Cd47-645M
Product Overview : Recombinant Mouse Cd47(Gln19-Pro158) fused with Fc of Human IgG1 at C-terminal was expressed in NS0.
Source : NS0
Species : Mouse
Tag : Fc
Predicted N Terminal : Gln19 predicted, no results obtained, sequencing might be blocked
Form : Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity : Measured by its binding ability in a functional ELISA. When Recombinant Mouse SIRP alpha /CD172a Fc Chimera is coated at 2 μg/mL, Recombinant Mouse CD47 Fc Chimera binds with an apparent KD <0.6 nM.Also measured by its ability to antagonize mouse red blood cell adhesion to immobilized Recombinant Mouse SIRP alpha /CD172a Fc Chimera. The ED50 for this effect is typically 2.0-8.0 μg/mL.Optimal dilutions should be determined by each laboratory for each application.
Molecular Mass : Predicted Molecular Mass: 42.3 (monomer) kDa;SDS-PAGE: 60-70 kDa, reducing conditions
Protein length : Gln19-Pro158
Endotoxin : <0.01 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 200 μg/mL in PBS.
Shipping : The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Gene Name : Cd47 CD47 antigen (Rh-related antigen, integrin-associated signal transducer) [ Mus musculus ]
Official Symbol : Cd47
Synonyms : CD47; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); leukocyte surface antigen CD47; Rh-related antigen; integrin-associated protein; IAP; Itgp; AA407862; AI848868; AW108519; 9130415E20Rik; B430305P08Rik;
Gene ID : 16423
mRNA Refseq : NM_010581
Protein Refseq : NP_034711
UniProt ID : Q61735
Chromosome Location : 16; 16 B5
Pathway : Cell surface interactions at the vascular wall, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; Hemostasis, organism-specific biosystem; Signal regulatory protein (SIRP) family interactions, organism-specific biosystem;
Function : protein binding; thrombospondin receptor activity;

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