Active Recombinant Mouse Wnt10b

• Cat.No.: Wnt10b-664M
• Product Overview: Recombinant Mouse Wnt10b (Accession # NP_035848) Met1-Lys389, fused with , was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Mouse
• Predicted N Terminal: Asn29
• Form: Lyophilized from a 0.2 µm filtered solution in PBS, NaCl, EDTA and CHAPS with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation/survival assay using IEC-18 rat small intestinal epithelial cells.The ED50 for this effect is typically 0.3-1.2 µg/mL.
• Molecular Mass: Recombinant Mouse Wnt10b has a calculated MW of 40.1 kDa. In SDS-PAGE migrates as 42 kDa, reducing conditions.
• Protein length: Met1-Lys389
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Wnt10b wingless related MMTV integration site 10b [ Mus musculus ]
• Official Symbol: Wnt10b
• Synonyms: WNT10B; wingless related MMTV integration site 10b; protein Wnt-10b; wnt-12; Wnt12
• Gene ID: 22410
• mRNA Refseq: NM_011718
• Protein Refseq: NP_035848