Recombinant Mouse Gdf7 protein Gdf7-675M

Recombinant Mouse Gdf7 protein

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Recombinant Mouse Gdf7 protein

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Cat.No. : Gdf7-675M
Product Overview : Recombinant Mouse Gdf7 protein was expressed in Escherichia coli.
Description : Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.
Source : E.coli
Species : Mouse
Form : Lyophilized from a 0.2μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2000 IU/mg.
Molecular Mass : Approximately 29.8 kDa, a homodimeric protein consisting of two 146 amino acid non-glycosylated polypeptide chains.
Protein length : 146
AA Sequence : TALAGTRGAQGSGGG GGGGGGGGGGGGGGG GGAGRGHGRRGRSRC SRKSLHVDFKELGWD DWIIAPLDYEAYHCE GVCDFPLRSHLEPTN HAIIQTLLNSMAPDA APASCCVPARLSPIS ILYIDAANNVVYKQY EDMVVEACGCR
Endotoxin : Less than 0.1 EU/µg of rMuGDF-7/BMP-12 as determined by LAL method.
Purity : >95% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
SDS-PAGE of Gdf7-675M :

Gene Name : Gdf7
Official Symbol : Gdf7
Synonyms : GDF7; growth differentiation factor 7; growth/differentiation factor 7; GDF-7; BMP12;
Gene ID : 238057
mRNA Refseq : NM_013527
Protein Refseq : NP_038555
UniProt ID : P43029

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