Active Recombinant Mouse Madcam1 Protein, Fc Chimera

• Cat.No.: Madcam1-676M
• Product Overview: Recombinant Mouse Madcam1 (Accession # NP_038619), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: Fc
• Predicted N Terminal: No results obtained: Gln22 predicted
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of HuT 78 human cutaneous T cell lymphoma cells.When 5 x 10^4 cells/well are added to mouse MAdCAM-1/Fc coated plates (10 µg/mL with 100 µL/well), approximately 80 - 90% will adhere after MnCl2 stimulation for 1 hour incubation at room temperature.Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Mouse Madcam1, Fc Chimera has a calculated MW of 63 kDa (monomer). In SDS-PAGE migrates as 85-95 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Madcam1 mucosal vascular addressin cell adhesion molecule 1 [ Mus musculus ]
• Official Symbol: Madcam1
• Synonyms: MADCAM1; mucosal vascular addressin cell adhesion molecule 1; mucosal addressin cell adhesion molecule 1; MAdCAM-1; mMAdCAM-1; AV211525; MGC35998; MGC144710; MGC144711
• Gene ID: 17123
• mRNA Refseq: NM_013591
• Protein Refseq: NP_038619