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Recombinant Human DKK4 Protein, His-tagged

Cat.No.: DKK4-681H
Product Overview: Recombinant Human DKK4 (Accession # NP_055235) Leu19-Leu224, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO Cells
Species: Human
Tag: His
Predicted N Terminal: Leu19
Form: Lyophilized from a 0.2 µm filtered solution in Tris-Citrate and NaCl with BSA as a carrier protein.
Bio-activity: Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. Hsieh, S.Y. et al. (2004) Oncogene 23:9183.The ED50 for this effect is typically 1 - 5 µg/mL. Optimal concentrations should be determined by each laboratory for each application.
Molecular Mass: Recombinant Human DKK4, His-tagged has a calculated MW of 24.4 kDa. In SDS-PAGE migrates as 35 kDa, reducing conditions.
Protein length: Leu19-Leu224
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: DKK4 dickkopf homolog 4 (Xenopus laevis) [ Homo sapiens ]
Official Symbol: DKK4
Synonyms: DKK4; dickkopf homolog 4 (Xenopus laevis); dickkopf (Xenopus laevis) homolog 4; dickkopf-related protein 4; hDkk-4; dickkopf-4; DKK-4; MGC129562; MGC129563;
Gene ID: 27121
mRNA Refseq: NM_014420
Protein Refseq: NP_055235
MIM: 605417
UniProt ID: Q9UBT3
Chromosome Location: 8p11.2-p11.1

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