Active Recombinant Human DKK4 Protein, His-tagged

• Cat.No.: DKK4-681H
• Product Overview: Recombinant Human DKK4 (Accession # NP_055235) Leu19-Leu224, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Leu19
• Form: Lyophilized from a 0.2 µm filtered solution in Tris-Citrate and NaCl with BSA as a carrier protein.
• Bio-activity: Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. Hsieh, S.Y. et al. (2004) Oncogene 23:9183.The ED50 for this effect is typically 1 - 5 µg/mL. Optimal concentrations should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human DKK4, His-tagged has a calculated MW of 24.4 kDa. In SDS-PAGE migrates as 35 kDa, reducing conditions.
• Protein length: Leu19-Leu224
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: DKK4 dickkopf homolog 4 (Xenopus laevis) [ Homo sapiens ]
• Official Symbol: DKK4
• Synonyms: DKK4; dickkopf homolog 4 (Xenopus laevis); dickkopf (Xenopus laevis) homolog 4; dickkopf-related protein 4; hDkk-4; dickkopf-4; DKK-4; MGC129562; MGC129563
• Gene ID: 27121
• mRNA Refseq: NM_014420
• Protein Refseq: NP_055235
• MIM: 605417
• UniProt ID: Q9UBT3
• Chromosome Location: 8p11.2-p11.1