Active Recombinant Mouse Cdh13 Protein, His-tagged

• Cat.No.: Cdh13-721M
• Product Overview: Recombinant Mouse Cdh13 (Accession # NP_062681) Met1-Gly693, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Ala22
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to inhibit the adhesion of HUVEC human umbilical vein endothelial cells.When 5 x 10^4 cells/well are added to Recombinant Human Cadherin-13 coated plates, cell adhesion is inhibited in a dose dependent manner after 2.5 hours at 37 °C. The ED50 for this effect is typically 1.0 - 5.0 µg/mL.
• Molecular Mass: Recombinant Mouse Cdh13, His-tagged has a calculated MW of 74.4 kDa. In SDS-PAGE migrates as 100-120 kDa, reducing conditions.
• Protein length: Met1-Gly693
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Cdh13 cadherin 13 [ Mus musculus ]
• Official Symbol: Cdh13
• Synonyms: CDH13; cadherin 13; cadherin-13; T-cad; H-cadherin; heart cadherin; Cdht; Tcad; T-cadherin; 4932416G01Rik
• Gene ID: 12554
• mRNA Refseq: NM_019707
• Protein Refseq: NP_062681