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Recombinant Human CD276 Protein, His-tagged

Cat.No.: CD276-757H
Product Overview: Recombinant Human CD276 (Accession # NP_079516) Leu29-Pro245, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO Cells
Species: Human
Tag: His
Predicted N Terminal: Leu29
Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Bio-activity: Measured in a cell proliferation assay using mouse CD4+ cells in the presence of anti-CD3.The ED50 for this effect is typically 3-12 µg/mL.
Molecular Mass: Recombinant Human CD276, His-tagged has a calculated MW of 24.8 kDa. In SDS-PAGE migrates as 40-45 kDa, reducing conditions.
Protein length: Leu29-Pro245
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution: Reconstitute at 25 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name: CD276 CD276 molecule [ Homo sapiens ]
Official Symbol: CD276
Synonyms: CD276; CD276 molecule; CD276 antigen; B7 H3; B7H3; B7RP 2; B7 homolog 3; costimulatory molecule; B7-H3; B7RP-2; 4Ig-B7-H3;
Gene ID: 80381
mRNA Refseq: NM_001024736
Protein Refseq: NP_001019907
MIM: 605715
UniProt ID: Q5ZPR3
Chromosome Location: 15q23-q24

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