Recombinant Human CD276 Protein, His-tagged CD276-757H

Recombinant Human CD276 Protein, His-tagged

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Recombinant Human CD276 Protein, His-tagged

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Cat.No. : CD276-757H
Product Overview : Recombinant Human CD276 (Accession # NP_079516) Leu29-Pro245, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO Cells
Species : Human
Tag : His
Predicted N Terminal : Leu29
Form : Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Bio-activity : Measured in a cell proliferation assay using mouse CD4+ cells in the presence of anti-CD3.The ED50 for this effect is typically 3-12 µg/mL.
Molecular Mass : Recombinant Human CD276, His-tagged has a calculated MW of 24.8 kDa. In SDS-PAGE migrates as 40-45 kDa, reducing conditions.
Protein length : Leu29-Pro245
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 25 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : CD276 CD276 molecule [ Homo sapiens ]
Official Symbol : CD276
Synonyms : CD276; CD276 molecule; CD276 antigen; B7 H3; B7H3; B7RP 2; B7 homolog 3; costimulatory molecule; B7-H3; B7RP-2; 4Ig-B7-H3;
Gene ID : 80381
mRNA Refseq : NM_001024736
Protein Refseq : NP_001019907
MIM : 605715
UniProt ID : Q5ZPR3
Chromosome Location : 15q23-q24

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