Recombinant Mouse Prss32 Protein, His-tagged Prss32-760M

Recombinant Mouse Prss32 Protein, His-tagged

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Recombinant Mouse Prss32 Protein, His-tagged

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Cat.No. : Prss32-760M
Product Overview : Recombinant Mouse Prss32 (Accession # NP_081496) Ser20-Arg304, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO Cells
Species : Mouse
Tag : His
Predicted N Terminal : Ser20
Form : Lyophilized from a 0.2 µm filtered solution in MES and NaCl.
Bio-activity : Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Arg-ThioBenzyl ester (Z-R-SBzl), in the presence of 5,5"Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.The specific activity is >400 pmol/min/µg, as measured under the described conditions.
Molecular Mass : Recombinant Mouse Prss32, His-tagged has a calculated MW of 32 kDa. In SDS-PAGE migrates as 40 kDa doublet, reducing conditions.
Protein length : Ser20-Arg304
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 10 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : Prss32 protease, serine 32 [ Mus musculus (house mouse) ]
Official Symbol : Prss32
Synonyms : mT5; tryptase-5; 2010001P08Rik; protease, serine, 32; protease, serine, 32; eosinophil serine protease; testisin; tryptase 5
Gene ID : 69814
mRNA Refseq : NM_027220.2
Protein Refseq : NP_081496.2 
MIM :
Chromosome Location : 17 A3.3; 17 12.09 cM

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