Recombinant Mouse Prss32 Protein, His-tagged


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Recombinant Mouse Prss32 Protein, His-tagged

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Cat.No. : Prss32-760M
Product Overview : Recombinant Mouse Prss32 (Accession # NP_081496) Ser20-Arg304, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : Mammalian cells
Species : Mouse
Tag : His
Predicted N Terminal : Ser20
Form : Lyophilized from a 0.2 µm filtered solution in MES and NaCl.
Bio-activity : Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Arg-ThioBenzyl ester (Z-R-SBzl), in the presence of 5,5"Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.The specific activity is >400 pmol/min/µg, as measured under the described conditions.
Molecular Mass : Recombinant Mouse Prss32, His-tagged has a calculated MW of 32 kDa. In SDS-PAGE migrates as 40 kDa doublet, reducing conditions.
Protein length : Ser20-Arg304
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 10 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : Prss32 protease, serine 32 [ Mus musculus (house mouse) ]
Official Symbol : Prss32
Synonyms : mT5; tryptase-5; 2010001P08Rik; protease, serine, 32; protease, serine, 32; eosinophil serine protease; testisin; tryptase 5
Gene ID : 69814
mRNA Refseq : NM_027220.2
Protein Refseq : NP_081496.2 
Chromosome Location : 17 A3.3; 17 12.09 cM
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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