Active Recombinant Mouse Crisp4 Protein, His-tagged

• Cat.No.: Crisp4-770M
• Product Overview: Recombinant Mouse Crisp4 (Accession # NP_084309) Pro22-Lys250, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Leu23
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of NTera-2 human testicular embryonic carcinoma cells.The ED50 for this effect is typically 0.3 - 1.5 µg/mL after a 1 hour incubation at37 °C. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Mouse Crisp4, His-tagged has a calculated MW of 27.2 kDa. In SDS-PAGE migrates as 34-37 kDa, reducing conditions.
• Protein length: Pro22-Lys250
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 50 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Crisp4 cysteine-rich secretory protein 4 [ Mus musculus ]
• Official Symbol: Crisp4
• Synonyms: CRISP4; cysteine-rich secretory protein 4; cysteine-rich secretory protein 1; 9230112K08Rik
• Gene ID: 78081
• mRNA Refseq: NM_030033
• Protein Refseq: NP_084309