||Recombinant Human MAP1LC3A (Accession # NP_115903), fused with an N-terminal 6-His tag, was produced in E. coli.
||X mg/ml (XµM) in 50mM Hepes pH 7.5, 100mM NaCl.Actual concentration varies with lot number.
||Biotin-His6-LC3/MAP1LC3A/Apg8p3 can be conjugated to substrate proteins via the subsequent actions of a Apg-activating (E1) enzyme, a Apg-conjugating (E2) enzyme, and a Apg ligase (E3). Biotin-His6-LC3/MAP1LC3A/Apg8p3 is ideal for the visualization or quantification of thioester formation with avidin-linked reagents. Reaction conditions will need to be optimized for each specific application. We recommend an initial Biotin-His6-LC3/MAP1LC3A/Apg8p3 concentration of 1-5 µM.
||>95%, by PAGE.
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).