Active Recombinant Human ARTN

• Cat.No.: ARTN-801H
• Product Overview: Recombinant Human ARTN (Accession # NP_476432) Ala108-Gly220, fused with , was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Predicted N Terminal: Ala108
• Form: Lyophilized from a 0.2 µm filtered solution in HCl with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells.The ED50 for this effect is typically 4-16 ng/mL.Measured by its binding ability in a functional ELISA.Immobilized Recombinant Human GFR alpha -3/GDNF R alpha -3 Fc Chimera at 1 µg/mL (100 µL/well) can bind Recombinant Human Artemin with an apparent Kd <1 nM.
• Molecular Mass: Recombinant Human ARTN has a calculated MW of 12 kDa (monomer). In SDS-PAGE migrates as 12 kDa, reducing conditions.
• Protein length: Ala108-Gly220
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: ARTN artemin [ Homo sapiens ]
• Official Symbol: ARTN
• Synonyms: ARTN; artemin; ENOVIN; EVN; NBN; neublastin; neurotrophic factor
• Gene ID: 9048
• mRNA Refseq: NM_001136215
• Protein Refseq: NP_001129687
• MIM: 603886
• UniProt ID: Q5T4W7
• Chromosome Location: 1p33-p32