Recombinant Mouse Artn protein, hFc-tagged

• Cat.No.: Artn-853M
• Product Overview: Recombinant Mouse Artn protein(Q9Z0L2-1)(Ala112-Gly224), fused with N-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: Ala112-Gly224
• Tag: N-hFc
• Form: Lyophilized from 0.22μm filtered solution in 50mM Glycine,150mM NaCl (pH 3.2). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse ARTN, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse GFRA3, His Tag with the EC50 of 32.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 39.4 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM Glycine,150mM NaCl (pH 3.2).

Gene Information

• Gene Name: Artn artemin [ Mus musculus ]
• Official Symbol: Artn
• Synonyms: ARTN; artemin; neublastin
• Gene ID: 11876
• mRNA Refseq: NM_009711
• Protein Refseq: NP_033841