Recombinant Human UBE2F Protein, His-tagged UBE2F-803H

Recombinant Human UBE2F Protein, His-tagged

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Recombinant Human UBE2F Protein, His-tagged

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Cat.No. : UBE2F-803H
Product Overview : Recombinant Human UBE2F (Accession # NP_542409), fused with an N-terminal 6-His tag, was produced in E. coli.
Source : E. coli
Species : Human
Tag : His
Form : X mg/ml (X µM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Bio-activity : Recombinant Human His6-UBE2F is a member of the NEDD8-conjugating (E2) enzyme family that receives NEDD8 from a NEDD8-activating (E1) enzyme and subsequently interacts with a NEDD8 ligase (E3) to conjugate NEDD8 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2F concentration of 0.1-1 µM.
Molecular Mass : Recombinant Human UBE2F, His-tagged has a calculated MW of 23 kDa.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 50 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : UBE2F ubiquitin-conjugating enzyme E2F (UBC6 homolog, C. elegans) [ Homo sapiens ]
Official Symbol : UBE2F
Synonyms : UBE2F; ubiquitin-conjugating enzyme E2F (UBC6 homolog, C. elegans); NCE2; NEDD8 conjugating enzyme;
Gene ID : 23665
mRNA Refseq :
Protein Refseq :
MIM :
UniProt ID : Q969M7
Chromosome Location : 2q37.3

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