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Recombinant Mouse Dner Protein, Fc Chimera

Cat.No.: Dner-838M
Product Overview: Recombinant Mouse Dner (Accession # NP_690879), fused with , was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO Cells
Species: Mouse
Tag: Fc
Predicted N Terminal: Ala26
Form: Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity: Measured by its ability to inhibit Pleiotrophin-induced neurite outgrowth of E16-E18 rat embryonic cortical neurons.When 4 x 10^4 neurons/well are added to 96-well plate containing serial dilutions of Recombinant Mouse DNER Fc Chimera and immobilized Recombinant Mouse Pleiotrophin/PTN (1 µg/mL), neurite outgrowth is significantly inhibited in a dose dependent manner. The ED50 for this effect is typically 0.5 - 2.5 µg/mL.
Molecular Mass: Recombinant Mouse Dner, Fc Chimera has a calculated MW of 91.4 kDa (reduced). In SDS-PAGE migrates as 140-160 kDa, reducing conditions.
Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: Dner delta/notch-like EGF-related receptor [ Mus musculus ]
Official Symbol: Dner
Synonyms: DNER; delta/notch-like EGF-related receptor; delta and Notch-like epidermal growth factor-related receptor; transmembrane protein Bet; brain EGF repeat-containing transmembrane protein; BET; Bret; A930026D19Rik; MGC39059;
Gene ID: 227325
mRNA Refseq: NM_152915
Protein Refseq: NP_690879
MIM:

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