Active Recombinant Drosophila dpp

Cat.No. : dpp-843D
Product Overview : Recombinant Drosophila dpp (Accession # NP_722813) Asp457-Arg588, fused with an N-terminal Met, was produced in E. coli.
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Species : Drosophila
Source : E.coli
Protein Length : Asp457-Arg588
Predicted N Terminal : Met
Form : Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Bio-activity : Measured by its ability to induce Mad phosphorylation in S2 Drosophila cells transfected with Mad. Approximately 3 µg/mL of recombinant Drosophila DPP and higher can effectively induce Mad phosphorylation.Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351.The ED50 for this effect is typically 0.5 - 2 µg/mL.
Molecular Mass : Recombinant Drosophila dpp, Met-tagged has a calculated MW of 14.8 kDa (monomer).
Purity : >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name dpp decapentaplegic [ Drosophila melanogaster (fruit fly) ]
Official Symbol dpp
Synonyms blk; BMP; CG9885; Dm-DPP; Dmel\CG9885; Dpp; DPP; DPP-C; Hin-d; ho; l(2)10638; l(2)22Fa; l(2)k17036; M(2)23AB; M(2)LS1; shv; Tg; TGF-b; TGF-beta; TGFbeta; CG9885 gene product from transcript CG9885-RA; CG9885-PA; CG9885-PB; CG9885-PC; CG9885-PE; Decapentaplegic/Bone morphogenetic protein; Haplo-insufficientblink; bone morphogenetic protein; bone morphogenic protein; decapentaplegic; dpp-PA; dpp-PB; dpp-PC; dpp-PE; heldout; shortvein; tegula
Gene ID 33432
mRNA Refseq NM_057963.5
Protein Refseq NP_477311.1 
MIM
UniProt ID P07713
Chromosome Location 22F1-22F3; 2-4.0 cM

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