Active Recombinant Drosophila dpp

• Cat.No.: dpp-843D
• Product Overview: Recombinant Drosophila dpp (Accession # NP_722813) Asp457-Arg588, fused with an N-terminal Met, was produced in E. coli.

Specification

• Species: Drosophila
• Source: E.coli
• Protein Length: Asp457-Arg588
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce Mad phosphorylation in S2 Drosophila cells transfected with Mad. Approximately 3 µg/mL of recombinant Drosophila DPP and higher can effectively induce Mad phosphorylation.Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351.The ED50 for this effect is typically 0.5 - 2 µg/mL.
• Molecular Mass: Recombinant Drosophila dpp, Met-tagged has a calculated MW of 14.8 kDa (monomer).
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: dpp decapentaplegic [ Drosophila melanogaster (fruit fly) ]
• Official Symbol: dpp
• Synonyms: blk; BMP; CG9885; Dm-DPP; Dmel\CG9885; Dpp; DPP; DPP-C; Hin-d; ho; l(2)10638; l(2)22Fa; l(2)k17036; M(2)23AB; M(2)LS1; shv; Tg; TGF-b; TGF-beta; TGFbeta; CG9885 gene product from transcript CG9885-RA; CG9885-PA; CG9885-PB; CG9885-PC; CG9885-PE; Decapentaplegic/Bone morphogenetic protein; Haplo-insufficientblink; bone morphogenetic protein; bone morphogenic protein; decapentaplegic; dpp-PA; dpp-PB; dpp-PC; dpp-PE; heldout; shortvein; tegula
• Gene ID: 33432
• mRNA Refseq: NM_057963.5
• Protein Refseq: NP_477311.1
• UniProt ID: P07713
• Chromosome Location: 22F1-22F3; 2-4.0 cM