Active Recombinant Human TLR4 Protein, Ser & His-tagged

• Cat.No.: TLR4-886H
• Product Overview: Recombinant Human TLR4 (Accession # O00206) Glu24-Lys631, fused with a C-terminal Ser and 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: Glu24-Lys631
• Predicted N Terminal: Glu24
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA.When Recombinant Human (rh) TLR4 is Immobilized at 2 µg/mL (100 µL/well), the concentration of rhMD2 that produces 50% optimal binding response is found to be approximately 0.03 - 0.15 µg/mL.
• Molecular Mass: Recombinant Human TLR4, Ser & His-tagged has a calculated MW of 70.6 kDa. In SDS-PAGE migrates as 90-95 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: TLR4 toll-like receptor 4 [ Homo sapiens ]
• Official Symbol: TLR4
• Synonyms: TLR4; toll-like receptor 4; CD284; hToll; TLR 4; homolog of Drosophila toll; TOLL; TLR-4; ARMD10
• Gene ID: 7099
• mRNA Refseq: NM_003266
• Protein Refseq: NP_003257
• UniProt ID: O00206
• Chromosome Location: 9q33.1