Active Recombinant Human CYR61 Protein, Fc Chimera

• Cat.No.: CYR61-895H
• Product Overview: Recombinant Human CYR61 (Accession # O00622), fused with , was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Species: Human
• Source: CHO
• Tag: Fc
• Predicted N Terminal: Ala22
• Form: Lyophilized from a 0.2 µm filtered solution in PBS and NaCl.
• Bio-activity: Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. Ball, D.K. et al. (2003) Reproduction 125:271. Recombinant Human Cyr61/CCN1 Fc Chimera immobilized at 1.5 µg/mL, 100 µL/well, will mediate >25% BALB/3T3 cell adhesion (3 x 10^4 cell/well added).
• Molecular Mass: Recombinant Human CYR61, Fc Chimera has a calculated MW of 66.3 kDa. In SDS-PAGE migrates as 70-75 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: CYR61 cysteine-rich, angiogenic inducer, 61 [ Homo sapiens ]
• Official Symbol: CYR61
• Synonyms: CYR61; cysteine-rich, angiogenic inducer, 61; IGFBP10; protein CYR61; CCN1; GIG1; IBP-10; IGFBP-10; CCN family member 1; IGF-binding protein 10; cysteine-rich, anigogenic inducer, 61; cysteine-rich heparin-binding protein 61; insulin-like growth factor-binding protein 10
• Gene ID: 3491
• mRNA Refseq: NM_001554
• Protein Refseq: NP_001545
• MIM: 602369
• UniProt ID: O00622
• Chromosome Location: 1p22.3