||Recombinant Human UBE2C (Accession # O00762), fused with , was produced in E. coli.
||0.50 mg/ml (25 µM) in 50 mM HEPES pH 7.0, 200 mM NaCl, 10% Gycerol (v/v), 1 mM TCEP
||Recombinant Human UbcH10/UBE2C is a member of the Ubiquitinconjugating Enzyme (E2) family that receives Ubiquitin from a UbiquitinActivating Enzyme (E1) and subsequently interacts with a Ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial UbcH10/UBE2C concentration of 0.1-1 µM.
||Recombinant Human UBE2C has a calculated MW of 20 kDa.
||>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).