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Recombinant Human UBE2C

Cat.No.: UBE2C-900H
Product Overview: Recombinant Human UBE2C (Accession # O00762), fused with , was produced in E. coli.
Source: E. coli
Species: Human
Form: 0.50 mg/ml (25 µM) in 50 mM HEPES pH 7.0, 200 mM NaCl, 10% Gycerol (v/v), 1 mM TCEP
Bio-activity: Recombinant Human UbcH10/UBE2C is a member of the Ubiquitin­conjugating Enzyme (E2) family that receives Ubiquitin from a Ubiquitin­Activating Enzyme (E1) and subsequently interacts with a Ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial UbcH10/UBE2C concentration of 0.1-­1 µM.
Molecular Mass: Recombinant Human UBE2C has a calculated MW of 20 kDa.
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: UBE2C ubiquitin-conjugating enzyme E2C [ Homo sapiens ]
Official Symbol: UBE2C
Synonyms: UBE2C; ubiquitin-conjugating enzyme E2C; ubiquitin-conjugating enzyme E2 C; UBCH10; ubiquitin-protein ligase C; ubiquitin carrier protein C; ubiquitin carrier protein E2-C; cyclin-selective ubiquitin carrier protein; mitotic-specific ubiquitin-conjugating enzyme; dJ447F3.2;
Gene ID: 11065
mRNA Refseq: NM_007019
Protein Refseq: NP_008950
MIM: 605574
UniProt ID: O00762
Chromosome Location: 20q13.12

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