Active Recombinant Human UBE2L6 Protein, GST-tagged

• Cat.No.: UBE2L6-928H
• Product Overview: Recombinant Human UBE2L6 (Accession # O14933), fused with an N-terminal GST (glutathione S-transferase) tag, was produced in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Form: X mg/ml (X µM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
• Bio-activity: Recombinant Human GST-UbcH8/UBE2L6 is a member of the Ub AND ISG15-conjugating (E2) enzyme family that receives Ub AND ISG15 from a Ub AND ISG15-activating (E1) enzyme and subsequently interacts with a Ub AND ISG15 ligase (E3) to conjugate Ub AND ISG15 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-UbcH8/UBE2L6 concentration of 0.1-1 µM.
• Molecular Mass: Recombinant Human UBE2L6, GST-tagged has a calculated MW of 44 kDa.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: UBE2L6 ubiquitin-conjugating enzyme E2L 6 [ Homo sapiens ]
• Official Symbol: UBE2L6
• Synonyms: UBE2L6; ubiquitin-conjugating enzyme E2L 6; ubiquitin/ISG15-conjugating enzyme E2 L6; UBCH8; ubiquitin-protein ligase L6; ubiquitin carrier protein L6; retinoic acid induced gene B protein; retinoic acid-induced gene B protein; RIG-B; MGC40331
• Gene ID: 9246
• mRNA Refseq: NM_004223
• Protein Refseq: NP_004214
• MIM: 603890
• UniProt ID: O14933
• Chromosome Location: 11q12.1