||Recombinant Human UBE2L6 (Accession # O14933), fused with , was produced in E. coli.
||X mg/ml (X µM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
||Recombinant Human UbcH8/UBE2L6 is a member of the Ub AND ISG15-conjugating (E2) enzyme family that receives Ub AND ISG15 from a Ub AND ISG15-activating (E1) enzyme and subsequently interacts with a Ub AND ISG15 ligase (E3) to conjugate Ub AND ISG15 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH8/UBE2L6 concentration of 0.1-1 µM.
||Recombinant Human UBE2L6 has a calculated MW of 18 kDa.
||>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).