Recombinant Human SEMA3E Protein, His-tagged SEMA3E-932H

Recombinant Human SEMA3E Protein, His-tagged

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Recombinant Human SEMA3E Protein, His-tagged

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Cat.No. : SEMA3E-932H
Product Overview : Recombinant Human SEMA3E (Accession # O15041) Thr25-Ser775, fused with an N-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO Cells
Species : Human
Tag : His
Predicted N Terminal : His
Form : Lyophilized from a 0.2 µm filtered solution in PBS and Tween® 20.
Bio-activity : Measured by its binding ability in a functional ELISA.When Recombinant Human Plexin D1 is coated at 5 µg/mL, Recombinant Human Semaphorin 3E binds with an apparent KD <2 nM.Measured by its ability to inhibit the proliferation of HUVEC human umbilical vein endothelial cells. Moriya, J. et al. (2010) Circ. Res. 10^6:391.The ED50 for this effect is typically 0.3-1.5 µg/mL.
Molecular Mass : Recombinant Human SEMA3E, His-tagged has a calculated MW of 87.9 kDa (monomer). In SDS-PAGE migrates as 90 kDa, 66 kDa and 25 kDa, reducing conditions.
Protein length : Thr25-Ser775
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : SEMA3E sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E [ Homo sapiens ]
Official Symbol : SEMA3E
Synonyms : SEMA3E; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E; SEMAH; semaphorin-3E; coll 5; KIAA0331; M sema H; M SemaK; coll-5; M-SEMAH; M-SemaK;
Gene ID : 9723
mRNA Refseq : NM_001178129
Protein Refseq : NP_001171600
MIM : 608166
UniProt ID : O15041
Chromosome Location : 7q21.11

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