Recombinant Human SEMA3E Protein, His-tagged


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Recombinant Human SEMA3E Protein, His-tagged

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Cat.No. : SEMA3E-932H
Product Overview : Recombinant Human SEMA3E (Accession # O15041) Thr25-Ser775, fused with an N-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : Mammalian cells
Species : Human
Tag : His
Predicted N Terminal : His
Form : Lyophilized from a 0.2 µm filtered solution in PBS and Tween® 20.
Bio-activity : Measured by its binding ability in a functional ELISA.When Recombinant Human Plexin D1 is coated at 5 µg/mL, Recombinant Human Semaphorin 3E binds with an apparent KD <2 nM.Measured by its ability to inhibit the proliferation of HUVEC human umbilical vein endothelial cells. Moriya, J. et al. (2010) Circ. Res. 10^6:391.The ED50 for this effect is typically 0.3-1.5 µg/mL.
Molecular Mass : Recombinant Human SEMA3E, His-tagged has a calculated MW of 87.9 kDa (monomer). In SDS-PAGE migrates as 90 kDa, 66 kDa and 25 kDa, reducing conditions.
Protein length : Thr25-Ser775
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : SEMA3E sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E [ Homo sapiens ]
Official Symbol : SEMA3E
Synonyms : SEMA3E; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E; SEMAH; semaphorin-3E; coll 5; KIAA0331; M sema H; M SemaK; coll-5; M-SEMAH; M-SemaK;
Gene ID : 9723
mRNA Refseq : NM_001178129
Protein Refseq : NP_001171600
MIM : 608166
UniProt ID : O15041
Chromosome Location : 7q21.11
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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