Active Recombinant Human SEMA3E Protein, His-tagged

• Cat.No.: SEMA3E-932H
• Product Overview: Recombinant Human SEMA3E (Accession # O15041) Thr25-Ser775, fused with an N-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: His
• Form: Lyophilized from a 0.2 µm filtered solution in PBS and Tween® 20.
• Bio-activity: Measured by its binding ability in a functional ELISA.When Recombinant Human Plexin D1 is coated at 5 µg/mL, Recombinant Human Semaphorin 3E binds with an apparent KD <2 nM.Measured by its ability to inhibit the proliferation of HUVEC human umbilical vein endothelial cells. Moriya, J. et al. (2010) Circ. Res. 10^6:391.The ED50 for this effect is typically 0.3-1.5 µg/mL.
• Molecular Mass: Recombinant Human SEMA3E, His-tagged has a calculated MW of 87.9 kDa (monomer). In SDS-PAGE migrates as 90 kDa, 66 kDa and 25 kDa, reducing conditions.
• Protein length: Thr25-Ser775
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: SEMA3E sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E [ Homo sapiens ]
• Official Symbol: SEMA3E
• Synonyms: SEMA3E; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E; SEMAH; semaphorin-3E; coll 5; KIAA0331; M sema H; M SemaK; coll-5; M-SEMAH; M-SemaK
• Gene ID: 9723
• mRNA Refseq: NM_001178129
• Protein Refseq: NP_001171600
• MIM: 608166
• UniProt ID: O15041
• Chromosome Location: 7q21.11