Active Recombinant Human DHH

• Cat.No.: DHH-967H
• Product Overview: Recombinant Human DHH (Accession # O43323) Cys23-Gly198 (Cys23Ile-Ile), fused with with and without an N-terminal Met, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Predicted N Terminal: Met & Ile-Ile
• Form: Lyophilized from a 0.2 µm filtered solution in NaH2PO4, NaCl and DTT .
• Bio-activity: Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465.The ED50 for this effect is typically 15 - 45 µg/mL.
• Molecular Mass: Recombinant Human DHH, Met-tagged has a calculated MW of 20 kDa.
• Protein length: Cys23-Gly198 (Cys23Ile-Ile)
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: DHH desert hedgehog [ Homo sapiens ]
• Official Symbol: DHH
• Synonyms: DHH; desert hedgehog; desert hedgehog (Drosophila) homolog; desert hedgehog protein; HHG 3; MGC35145; mutant desert hedgehog; desert hedgehog homolog; GDXYM; HHG-3; SRXY7
• Gene ID: 50846
• mRNA Refseq: NM_021044
• Protein Refseq: NP_066382
• MIM: 605423
• UniProt ID: O43323
• Chromosome Location: 12q13.1