Active Recombinant Mouse Dkk1

• Cat.No.: Dkk1-985M
• Product Overview: Recombinant Mouse Dkk1 (Accession # O54908) Ser30-His272, fused with , was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Mouse
• Predicted N Terminal: Ser30 & Thr32
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.The ND50 for this effect is typically 0.02-0.1 µg/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt-3a.
• Molecular Mass: Recombinant Mouse Dkk1 has a calculated MW of 26.1 kDa. In SDS-PAGE migrates as 35-40 kDa, reducing conditions.
• Protein length: Ser30-His272
• Purity: >95%, by SDS-PAGE with silver staining.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Dkk1 dickkopf homolog 1 (Xenopus laevis) [ Mus musculus ]
• Official Symbol: Dkk1
• Synonyms: DKK1; dickkopf homolog 1 (Xenopus laevis); dickkopf-related protein 1; dkk-1; dickkopf-1; mdkk-1
• Gene ID: 13380
• mRNA Refseq: NM_010051
• Protein Refseq: NP_034181