Active Recombinant Rat Robo1 Protein, Fc Chimera

• Cat.No.: Robo1-988R
• Product Overview: Recombinant Rat Robo1 (Accession # O55005), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Rat
• Tag: Fc
• Predicted N Terminal: Lys19
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA.Biotinylated recombinant mouse Slit-3 immobilized on a streptavidin-coated plate at 1 µg/mL can bind Recombinant Rat ROBO1 Fc Chimera with an apparent Kd <10 nM.
• Molecular Mass: Recombinant Rat Robo1, Fc Chimera has a calculated MW of 86.2 kDa (monomer). In SDS-PAGE migrates as 110-115 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 50 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Robo1 roundabout homolog 1 (Drosophila) [ Rattus norvegicus ]
• Official Symbol: Robo1
• Synonyms: ROBO1; roundabout homolog 1 (Drosophila); roundabout homolog 1
• Gene ID: 58946
• mRNA Refseq: NM_022188
• Protein Refseq: NP_071524