Recombinant Human AKR1B10 Protein, Met & His-tagged AKR1B10-995H

Cat.No.:	AKR1B10-995H
Product Overview:	Recombinant Human AKR1B10 (Accession # O60218) Ala2-Tyr316, fused with an N-terminal Met and 6-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.
Source:	Baculovirus Sf21 Insect Cells
Species:	Human
Tag:	& His
Predicted N Terminal:	Inconclusive result, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments
Form:	Supplied as a 0.2 µm filtered solution in Tris, NaCl, DTT and Glycerol.
Bio-activity:	Measured by the disappearance of NADPH during the reducation of 4-nitrobenzaldehyde.The specific activity is >1,000 pmol/min/µg, as measured under the described conditions.
Molecular Mass:	Recombinant Human AKR1B10, Met & His-tagged has a calculated MW of 37 kDa. In SDS-PAGE migrates as 37-38 kDa, reducing conditions.
Protein length:	Ala2-Tyr316
Purity:	>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage:	Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution:	Reconstitute at 100 µg/mL in PBS containing at least 0.1% human or bovine serum albumin.

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Gene Name:	AKR1B10 aldo-keto reductase family 1, member B10 (aldose reductase) [ Homo sapiens ]
Official Symbol:	AKR1B10
Synonyms:	AKR1B10; aldo-keto reductase family 1, member B10 (aldose reductase); AKR1B11; aldo-keto reductase family 1 member B10; AKR1B12; aldo keto reductase family 1; member B11 (aldose reductase like); aldose reductase like 1; aldose reductase like peptide; aldose reductase related protein; ALDRLn; ARL 1; ARL1; HIS; HSI; small intestine reductase; ARP; hARP; SI reductase; aldose reductase-like 1; aldose reductase-like peptide; aldose reductase-related protein; aldo-keto reductase family 1, member B11 (aldose reductase-like); ARL-1; MGC14103;
Gene ID:	57016
mRNA Refseq:	NM_020299
Protein Refseq:	NP_064695
MIM:	604707
UniProt ID:	O60218
Chromosome Location:	7q33