Recombinant Human TPST2 Protein, His-tagged TPST2-1006H

Recombinant Human TPST2 Protein, His-tagged

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Recombinant Human TPST2 Protein, His-tagged

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Cat.No. : TPST2-1006H
Product Overview : Recombinant Human TPST2 (Accession # O60704) Gln26-Ser377, fused with an N-terminal CD33 signal sequence and a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source : CHO Cells
Species : Human
Tag : His
Predicted N Terminal : No N-terminal sequence was revealed by sequencing. The Gln residue maybe blocked. Protein identity confirmed by MS analysis of tryptic fragments.
Form : Supplied as a 0.2 µm filtered solution in Tris and NaCl.
Bio-activity : Measured by its ability to transfer sulfate from PAPS to PSGL-1 peptide (Gln-Ala-Thr-Glu-Tyr-Glu-Tyr-Leu-Asp-Tyr-Asp-Phe-Leu-Pro-Glu-Thr)The specific activity is >20 pmol/min/µg, as measured under the described conditions.
Molecular Mass : Recombinant Human TPST2, His-tagged has a calculated MW of 40 kDa. In SDS-PAGE migrates as 40-50 kDa, reducing conditions.
Protein length : Gln26-Ser377
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 100 µg/mL in sterile 4 mM HCI containing at least 0.1% human or bovine serum albumin.
Gene Name : TPST2 tyrosylprotein sulfotransferase 2 [ Homo sapiens ]
Official Symbol : TPST2
Synonyms : TPST2; tyrosylprotein sulfotransferase 2; protein-tyrosine sulfotransferase 2; TPST-2; tyrosylprotein sulfotransferase-2; tyrosylprotein phosphotransferase 2;
Gene ID : 8459
mRNA Refseq : NM_001008566
Protein Refseq : NP_001008566
MIM : 603126
UniProt ID : O60704
Chromosome Location : 22q12.1

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