Recombinant Human TPST2 Protein, His-tagged


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Recombinant Human TPST2 Protein, His-tagged

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Cat.No. : TPST2-1006H
Product Overview : Recombinant Human TPST2 (Accession # O60704) Gln26-Ser377, fused with an N-terminal CD33 signal sequence and a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source : CHO Cells
Species : Human
Tag : His
Predicted N Terminal : No N-terminal sequence was revealed by sequencing. The Gln residue maybe blocked. Protein identity confirmed by MS analysis of tryptic fragments.
Form : Supplied as a 0.2 µm filtered solution in Tris and NaCl.
Bio-activity : Measured by its ability to transfer sulfate from PAPS to PSGL-1 peptide (Gln-Ala-Thr-Glu-Tyr-Glu-Tyr-Leu-Asp-Tyr-Asp-Phe-Leu-Pro-Glu-Thr)The specific activity is >20 pmol/min/µg, as measured under the described conditions.
Molecular Mass : Recombinant Human TPST2, His-tagged has a calculated MW of 40 kDa. In SDS-PAGE migrates as 40-50 kDa, reducing conditions.
Protein length : Gln26-Ser377
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 100 µg/mL in sterile 4 mM HCI containing at least 0.1% human or bovine serum albumin.
Gene Name : TPST2 tyrosylprotein sulfotransferase 2 [ Homo sapiens ]
Official Symbol : TPST2
Synonyms : TPST2; tyrosylprotein sulfotransferase 2; protein-tyrosine sulfotransferase 2; TPST-2; tyrosylprotein sulfotransferase-2; tyrosylprotein phosphotransferase 2;
Gene ID : 8459
mRNA Refseq : NM_001008566
Protein Refseq : NP_001008566
MIM : 603126
UniProt ID : O60704
Chromosome Location : 22q12.1
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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