Active Recombinant Human ICOSLG Protein, Fc Chimera

• Cat.No.: ICOSLG-1026H
• Product Overview: Recombinant Human ICOSLG (Accession # O75144), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Predicted N Terminal: Asp19
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to stimulate human T cell proliferation in the presence of anti-CD3. Wang, S. et al. (2000) Blood 96:2808.The ED50 for this effect is 0.3-1.2 µg/mL in the presence of anti-CD3 immobilized at 20 ng/mL. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human ICOSLG, Fc Chimera has a calculated MW of 53.4 kDa (monomer). In SDS-PAGE migrates as 80-90 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: ICOSLG inducible T-cell co-stimulator ligand [ Homo sapiens ]
• Official Symbol: ICOSLG
• Synonyms: ICOSLG; inducible T-cell co-stimulator ligand; ICOSL; ICOS ligand; B7 homolog 2; B7 homologue 2; B7 H2; B7 related protein 1; B7H2; B7RP 1; B7RP1; CD275; GL50; ICOS L; KIAA0653; B7-like protein Gl50; B7-related protein 1; transmembrane protein B7-H2 ICOS ligand; B7-H2; LICOS; B7RP-1; ICOS-L
• Gene ID: 23308
• mRNA Refseq: NM_015259
• Protein Refseq: NP_056074
• MIM: 605717
• UniProt ID: O75144
• Chromosome Location: 21q22.3