Recombinant Human LGR5 Protein, Fc Chimera LGR5-1037H

Recombinant Human LGR5 Protein, Fc Chimera

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Recombinant Human LGR5 Protein, Fc Chimera

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Cat.No. : LGR5-1037H
Product Overview : Recombinant Human LGR5 (Accession # O75473), fused with , was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source : CHO Cells
Species : Human
Tag : Fc
Predicted N Terminal : Gly22
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by its binding ability in a functional ELISA.When Recombinant Human R-Spondin 3 is coated at 0.5 µg/mL, Recombinant Human Lgr5/GPR49 Fc Chimera binds with an apparent Kd <0.1 nM. Measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells.The ED50 for this effect is typically 1.5-7.5 µg/mL in the presence of 20 ng/mL Recombinant Human R-Spondin 2 and 5 ng/mL Recombinant Mouse Wnt-3a.
Molecular Mass : Recombinant Human LGR5, Fc Chimera has a calculated MW of 87 kDa (monomer). In SDS-PAGE migrates as 90-110 kDa, reducing conditions.
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : LGR5 leucine-rich repeat containing G protein-coupled receptor 5 [ Homo sapiens ]
Official Symbol : LGR5
Synonyms : LGR5; leucine-rich repeat containing G protein-coupled receptor 5; G protein coupled receptor 49 , GPR49, GPR67, leucine rich repeat containing G protein coupled receptor 5; leucine-rich repeat-containing G-protein coupled receptor 5; FEX; HG38; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38; orphan G protein-coupled receptor HG38; leucine-rich repeat-containing G protein-coupled receptor 5; GPR49; GPR67; GRP49; MGC117008;
Gene ID : 8549
mRNA Refseq : NM_003667
Protein Refseq : NP_003658
MIM : 606667
UniProt ID : O75473
Chromosome Location : 12q22-q23

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