Active Recombinant Human LRP6 Protein, Fc Chimera

• Cat.No.: LRP6-1040H
• Product Overview: Recombinant Human LRP6 (Accession # O75581), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Predicted N Terminal: Ala20
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA.rhLRP-6/Fc Chimera binds rhDkk-1, biotin with an apparent KD < 25 nM.Biotinylated rmWnt-3a immobilized on a streptavidin-coated plate at 1 µg/mL can bind rhLRP-6/Fc Chimera with a linear range of 0.2 - 10 µg/mL. Optimal concentrations should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human LRP6, Fc Chimera has a calculated MW of 180 kDa (monomer). In SDS-PAGE migrates as 200 kDa, reducing conditions.
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 10 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: LRP6 low density lipoprotein receptor-related protein 6 [ Homo sapiens ]
• Official Symbol: LRP6
• Synonyms: LRP6; low density lipoprotein receptor-related protein 6; low-density lipoprotein receptor-related protein 6; LRP-6; ADCAD2; FLJ90062; FLJ90421
• Gene ID: 4040
• mRNA Refseq: NM_002336
• Protein Refseq: NP_002327
• MIM: 603507
• UniProt ID: O75581
• Chromosome Location: 12p13.2