Recombinant Human ATG7 Protein, His-tagged


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Recombinant Human ATG7 Protein, His-tagged

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Cat.No. : ATG7-1076H
Product Overview : Recombinant Human ATG7 (Accession # O95352), fused with an N-terminal 6-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.
Source : Baculovirus Sf21 Insect Cells
Species : Human
Tag : His
Form : X mg/ml (X µM) in 50 mM HEPES pH 7.0, 150 mM NaCl, 2 mM TCEP, 10% glycerol
Bio-activity : Recombinant Human His6-ATG7 (Apg7) is a member of the Apg-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Apg-conjugating (E2) enzyme and a Apg ligase (E3) to conjugate Apg to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-ATG7 (Apg7) concentration of 50-200 nM.
Molecular Mass : Recombinant Human ATG7, His-tagged has a calculated MW of 80 kDa.
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : ATG7 ATG7 autophagy related 7 homolog (S. cerevisiae) [ Homo sapiens ]
Official Symbol : ATG7
Synonyms : ATG7; ATG7 autophagy related 7 homolog (S. cerevisiae); APG7 autophagy 7 like (S. cerevisiae) , APG7L; ubiquitin-like modifier-activating enzyme ATG7; DKFZp434N0735; GSA7; hAGP7; autophagy-related protein 7; ATG12-activating enzyme E1 ATG7; ubiquitin activating enzyme E1-like protein; ubiquitin-activating enzyme E1-like protein; APG7L; APG7-LIKE;
Gene ID : 10533
mRNA Refseq : NM_001136031
Protein Refseq : NP_001129503
MIM : 608760
UniProt ID : O95352
Chromosome Location : 3p25.3-p25.2
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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