Active Recombinant Human PGK1 Protein, His-tagged

• Cat.No.: PGK1-1102H
• Product Overview: Recombinant Human PGK1 (Accession # P00558) Met1-Ile417, fused with an C-terminal 10-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Source: Baculovirus Sf21 Insect Cells
• Species: Human
• Tag: His
• Predicted N Terminal: No results obtained
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.
• Bio-activity: Measured by NADH production in a reaction coupled with GAPDH.The specific activity, as measured under the described condtions, is >50,000 pmol/min/µg.
• Molecular Mass: Recombinant Human PGK1, His-tagged has a calculated MW of 46 kDa. In SDS-PAGE migrates as 42 kDa, reducing conditions.
• Protein length: Met1-Ile417
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: PGK1 phosphoglycerate kinase 1 [ Homo sapiens ]
• Official Symbol: PGK1
• Synonyms: PGK1; phosphoglycerate kinase 1; PRP 2; primer recognition protein 2; cell migration-inducing gene 10 protein; PGKA; MIG10; MGC8947; MGC117307; MGC142128
• Gene ID: 5230
• mRNA Refseq: NM_000291
• Protein Refseq: NP_000282
• MIM: 311800
• UniProt ID: P00558
• Chromosome Location: Xq13.3