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Recombinant Human F10 Protein, His-tagged

Cat.No.: F10-1105H
Product Overview: Recombinant Human F10 (Accession # P00742) Met1-Lys488, fused with a C-terminal 10-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.
Source: Baculovirus Sf21 Insect Cells
Species: Human
Tag: His
Predicted N Terminal: Phe80, Tyr84, & Ile235
Form: Lyophilized from a 0.2 µm filtered solution in MES, NaCl and CaCl2.
Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is >800 pmol/min/μg, as measured under the described conditions.
Molecular Mass: Recombinant Human F10, His-tagged has a calculated MW of 16.6 kDa & 30 kDa. In SDS-PAGE migrates as 12-15 kDa and 30-36 kDa, reducing conditions.
Protein length: Met1-Lys488
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: F10 coagulation factor X [ Homo sapiens ]
Official Symbol: F10
Synonyms: F10; coagulation factor X; factor Xa; prothrombinase; Stuart-Prower factor; FX; FXA;
Gene ID: 2159
mRNA Refseq: NM_000504
Protein Refseq: NP_000495
MIM: 613872
UniProt ID: P00742
Chromosome Location: 13q34

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