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Recombinant Human REN Protein, His-tagged

Cat.No.: REN-1111H
Product Overview: Recombinant Human REN (Accession # P00797) Leu24-Arg406, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO Cells
Species: Human
Tag: His
Predicted N Terminal: Leu24
Form: Supplied as a 0.2 µm filtered solution in MES and NaCl.
Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate, Arg-Glu(EDANS)-Ile-His-Pro-Phe-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys(dabcyl)-Arg. The specific activity is >20 pmol/min/µg, as measured under the described conditions.
Molecular Mass: Recombinant Human REN, His-tagged has a calculated MW of 44 kDa. In SDS-PAGE migrates as 44 kDa and 51 kDa, reducing conditions.
Protein length: Leu24-Arg406
Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: REN renin [ Homo sapiens ]
Official Symbol: REN
Synonyms: REN; renin; angiotensinogenase; renin precursor, renal; angiotensin-forming enzyme; HNFJ2; FLJ10761;
Gene ID: 5972
mRNA Refseq: NM_000537
Protein Refseq: NP_000528
MIM: 179820
UniProt ID: P00797
Chromosome Location: 1q32

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