Active Recombinant Human REN Protein, His-tagged

• Cat.No.: REN-1111H
• Product Overview: Recombinant Human REN (Accession # P00797) Leu24-Arg406, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Leu24
• Form: Supplied as a 0.2 µm filtered solution in MES and NaCl.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate, Arg-Glu(EDANS)-Ile-His-Pro-Phe-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys(dabcyl)-Arg. The specific activity is >20 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human REN, His-tagged has a calculated MW of 44 kDa. In SDS-PAGE migrates as 44 kDa and 51 kDa, reducing conditions.
• Protein length: Leu24-Arg406
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: REN renin [ Homo sapiens ]
• Official Symbol: REN
• Synonyms: REN; renin; angiotensinogenase; renin precursor, renal; angiotensin-forming enzyme; HNFJ2; FLJ10761
• Gene ID: 5972
• mRNA Refseq: NM_000537
• Protein Refseq: NP_000528
• MIM: 179820
• UniProt ID: P00797
• Chromosome Location: 1q32