||Recombinant Human Neuron Specific Enolase is expressed inE. colicontaining 433 amino acids 2-434 fused to an amino terminal hexahistidine tag. The NSE is purified by proprietary chromatographic techniques.
||NSE is the γγ isoform of the glycolytic enzyme enolase and is expressed primarily in neurons, in normal and neoplastic neuroendocrine cells. NSE is a highly soluble cytoplasmic protein that is readily secreted into the CSF and serum following tissue damage. NSE shows neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons and binds in a calcium-dependent manner to cultured neocortical neurons promoting cell survival.
||Sterile Filtered clear solution.
||Greater than 95% as determined by SDS-PAGE. Single band on Western Blot.
||Enolase 2 is supplied in 1x Laemmli Buffer (25 mM Tris-HCl pH6.8, 50 mM DTT, 1%(w/v) SDS, 0.1%(w/v) Bromophenol Blue and 2.5% Glycerol).
||Recombinant Human NSE can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments. The biological activity of this product has not yet been tested.
||Store at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C for longer periods of time. Please prevent freeze-thaw cycles.