Recombinant Human Enolase 2 (Gamma, Neuronal), His-tagged

• Cat.No.: ENO2-43H
• Product Overview: Recombinant Human Neuron Specific Enolase is expressed inE. colicontaining 433 amino acids 2-434 fused to an amino terminal hexahistidine tag. The NSE is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: ENO2-43H
• Description: NSE is the γγ isoform of the glycolytic enzyme enolase and is expressed primarily in neurons, in normal and neoplastic neuroendocrine cells. NSE is a highly soluble cytoplasmic protein that is readily secreted into the CSF and serum following tissue damage. NSE shows neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons and binds in a calcium-dependent manner to cultured neocortical neurons promoting cell survival.
• Source: Escherichia Coli.
• Physical Appearance: Sterile Filtered clear solution.
• Purity: Greater than 95% as determined by SDS-PAGE. Single band on Western Blot.
• Formulation: Enolase 2 is supplied in 1x Laemmli Buffer (25 mM Tris-HCl pH6.8, 50 mM DTT, 1%(w/v) SDS, 0.1%(w/v) Bromophenol Blue and 2.5% Glycerol).
• Applications: Recombinant Human NSE can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments. The biological activity of this product has not yet been tested.
• Stability: Store at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C for longer periods of time. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: ENO2 enolase 2 (gamma, neuronal) [ Homo sapiens ]
• Synonyms: ENO2; enolase 2 (gamma, neuronal); NSE; EC=4.2.1.11; Gamma-enolase;2-phospho-D-glycerate hydro-lyase; Neural enolase; Neuron-specific enolase; Enolase 2; neuron specific gamma enolase; 2-phospho-D-glycerate hydrolyase; Neuron-specific enolase; neurone-specific enolase
• Gene ID: 2026
• mRNA Refseq: NM_001975
• Protein Refseq: NP_001966
• MIM: 131360
• UniProt ID: P09104
• Chromosome Location: 12p13
• Pathway: Biosynthesis of alkaloids derived from histidine and purine; Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid; Biosynthesis of alkaloids derived from shikimate pathway; Biosynthesis of alkaloids derived from terpenoid and polyketide; Biosynthesis of phenylpropanoids; Biosynthesis of plant hormones; Biosynthesis of terpenoids and steroids; Glycolysis / Gluconeogenesis; Metabolic pathways; RNA degradation; Diabetes pathways; Metabolism of carbohydrates
• Function: lyase activity; magnesium ion binding; phosphopyruvate hydratase activity; protein heterodimerization activity; protein homodimerization activity