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Recombinant Human Enolase 2 (Gamma, Neuronal), His-tagged

Cat. No.: ENO2-43H
Product Overview: Recombinant Human Neuron Specific Enolase is expressed inE. colicontaining 433 amino acids 2-434 fused to an amino terminal hexahistidine tag. The NSE is purified by proprietary chromatographic techniques.
Description: NSE is the γγ isoform of the glycolytic enzyme enolase and is expressed primarily in neurons, in normal and neoplastic neuroendocrine cells. NSE is a highly soluble cytoplasmic protein that is readily secreted into the CSF and serum following tissue damage. NSE shows neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons and binds in a calcium-dependent manner to cultured neocortical neurons promoting cell survival.
Source: Escherichia Coli.
Physical Appearance: Sterile Filtered clear solution.
Purity: Greater than 95% as determined by SDS-PAGE. Single band on Western Blot.
Formulation: Enolase 2 is supplied in 1x Laemmli Buffer (25 mM Tris-HCl pH6.8, 50 mM DTT, 1%(w/v) SDS, 0.1%(w/v) Bromophenol Blue and 2.5% Glycerol).
Applications: Recombinant Human NSE can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments. The biological activity of this product has not yet been tested.
Stability: Store at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C for longer periods of time. Please prevent freeze-thaw cycles.
Gene Name: ENO2 enolase 2 (gamma, neuronal) [ Homo sapiens ]
Synonyms: ENO2; enolase 2 (gamma, neuronal); NSE; EC=4.2.1.11; Gamma-enolase;2-phospho-D-glycerate hydro-lyase; Neural enolase; Neuron-specific enolase; Enolase 2; neuron specific gamma enolase; 2-phospho-D-glycerate hydrolyase; Neuron-specific enolase; neurone-specific enolase
Gene ID: 2026
mRNA Refseq: NM_001975
Protein Refseq: NP_001966
MIM: 131360
UniProt ID: P09104
Chromosome Location: 12p13
Pathway: Biosynthesis of alkaloids derived from histidine and purine; Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid; Biosynthesis of alkaloids derived from shikimate pathway; Biosynthesis of alkaloids derived from terpenoid and polyketide; Biosynthesis of phenylpropanoids; Biosynthesis of plant hormones; Biosynthesis of terpenoids and steroids; Glycolysis / Gluconeogenesis; Metabolic pathways; RNA degradation; Diabetes pathways; Metabolism of carbohydrates
Function: lyase activity; magnesium ion binding; phosphopyruvate hydratase activity; protein heterodimerization activity; protein homodimerization activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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