Recombinant Human GNAI3 protein, His-tagged GNAI3-382H

Recombinant Human GNAI3 protein, His-tagged

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Recombinant Human GNAI3 protein, His-tagged

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Cat.No. : GNAI3-382H
Product Overview : Recombinant Human GNAI3 (1-354aa) fussed with His tag at N-terminal was expressed in E. coli.
Description : GNAI3 belongs to the G-alpha family and G (i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor-regulated K+General references: channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. This protein plays a role in cell division. GNAI3 has been shown to interact with RGS14, RIC8A, RGS18, S1PR1, RGS12, RGS16, RGS19, RGS10 and RGS5.
Source : E. coli
Species : Human
Tag : His
Form : Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 100mM NaCl
Molecular Mass : 43.0kDa (377aa), confirmed by MALDI-TOF
AA Sequence : MGSSHHHHHH SSGLVPRGSH MGSQYKVVVYSNT IQSIIAIIRA MGRLKIDFGE AARADDARQL FVLAGSAEEG VMTPELAGVI KRLWRDGGVQ ACFSRSREYQ LNDSASYYLNMGCTLSA EDKAAVERSK MIDRNLREDG EKAAKEVKLL LLGAGESGKS TIVKQMKIIH EDGYSEDECKDLDRISQSNY IPTQQDVLRT RVKTTGIVET HFTFKDLYFK MFDVGGQRSE RKKWIHCFEG VTAIIFCVAL SDYDLVLAED EEMNRMHESMKLFDSICNNK WFTETSIILF LNKKDLFEEK IKRSPLTICY PEYTGSNTYE EAAAYIQCQF EDLNRRKDTK EIYTHFTCAT DTKNVQFVFDAVTDVIIKNN LKECGLY
Purity : > 90% by SDS - PAGE
Storage : Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade.Avoid repeated freezing and thawing cycles.
Concentration : 0.5mg/ml (determined by Bradford assay)
Gene Name : GNAI3 guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 [ Homo sapiens ]
Official Symbol : GNAI3
Synonyms : 87U6; ARCND1; guanine nucleotide-binding protein G(k) subunit alpha; g(i) alpha-3
Gene ID : 2773
mRNA Refseq : NM_006496
Protein Refseq : NP_006487
MIM : 139370
UniProt ID : P08754
Chromosome Location : 1p13
Pathway : ADP signalling through P2Y purinoceptor 12, organism-specific biosystem; Activation of GABAB receptors, organism-specific biosystem; Adrenergic signaling in cardiomyocytes, organism-specific biosystem
Function : G-protein beta/gamma-subunit complex binding; GTP binding; GTPase activity

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